Probing the molecular determinants of fluorinase specificity.
نویسندگان
چکیده
Molecular determinants of FlA1 fluorinase specificity were probed using 5'-chloro-5'-deoxyadenosine (5'-ClDA) analogs as substrates and FlA1 active site mutants. Modifications at F213 or A279 residues are beneficial towards these modified substrates, including 5'-chloro-5'-deoxy-2-ethynyladenosine, ClDEA (>10-fold activity improvement), and conferred novel activity towards substrates not readily accepted by wild-type FlA1.
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ورودعنوان ژورنال:
- Chemical communications
دوره 53 17 شماره
صفحات -
تاریخ انتشار 2017